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To: Washington m4 From: Washington Field Re: 279A-WF-222936-USAMRIID, 1/11/2006 antifoam emulsion did not interfere with the function of the cipritube jets. Technicians were careful to add minimal amounts of the antifoam emulsion, as the addition of excessive antifoam emulsion to the nebulizer increased the viscosity of the biological material to the point of interfere j bIF aerosolization. [peed that up to emulsion may have been uséd in the nebulizer during some plague aerosol challenges. For anthrax challenges, the AGI collection solution was emulsion was not a necessary addition to the AGI for anthrax OTF experiments, as the water did not bubble enough to disrupt the impingement process. oy JN The general SOP for conducting an aerosol challenge contained instructions for the use of antifoam emulsion; however, the SOP did not mention the option to use olive oil. An enlisted person ordered the antifoam through the USAMRIID supply system. The consistency of the antifoam was very thick and similar to that of mayonnaise. Due to the high viscosity of the antifoam, it was difficult to pipette. Asa result, the antifoam was added to the PBS. The antifoam emulsion was mixed by estimation and not measured exactly. Antifoam was never used in the concentrated form during aerosol challenges. The dilution of the antifoam with PBS was done in the — laminar flow hoods in roo The diluted antifoam_emulsion — was stored in an amber bottle in room[__lof BuildingL__] The — aerosol technicians sometimes made a large volume of the antifoam emulsion, which was stable for weeks. Eventually, the antifoam separated from the rest of the solution; however, shaking the bottle re-mixed the solution. b2 b7E OLIVE OIL AS AN ALTERNATIVE TO ANTIFOAM: 23