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4 To: Washington m1 From: Washington Field Re: 279A-WF-222936-USAMRIID, 1/11/2006 added to the AGI, it settled to the top of the solution. The purpose of antifoam was to prevent bubbling inside the nebulizer or AGI which caused poor aerosolization, or material loss when the material stuck to the sides of the container. The primary aerosol technicians were questioned about the use of antifoam as part of the aerosol process. All commented that it was not standard operating procedure (SOP) to utilize antifoam with Ba. Because the Ba aerosols used only water in the process, there was not usually enough foam created to require the use of antifoam. b2 The only documented uses of antifoam during Ba Ames aerosol challenges were on 6/26/2001 and 8/14/2001. Both of these challenges were conducted as part of Protocol B01-07 with as the PI for the study. The aerosol technicians who ran the challenges were[___Jon 6/26/2001 and[___| on 8/14/2001. When questioned about the instances, both technicians commented that antifoam was -utilized at the request off ta not recall requesting the use of antifoam. ° be b7C 21