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To: Washington m. From: Washington Field Re: 279A-WF-222936-USAMRIID, 1/11/2006 The option of using olive oil as a substitute for antifoam was not common_knowledge at USAMRIID. learned from[___] that[__] could use olive oil instead o antifoam. There are several accounts of bottles of Bertolli and Pompei olive oil being maintained on the hot side of Building cl in one of the aerosol rooms. stated that a ottle of Bertolli olive oil was kept in € preparation rooms used by the Aerobiology group in Building Do sed extra virgin olive oil; however, the brand or type of olive oil did not matter. There was not a particular reason as to why extra virgin olive oil was used. [J notea that olive oil could be used instead of antifoam and was sometimes used in the AGIs at USAMRIID. No _ documentation was found to suggest that olive oil was ever used in an Ames aerosol challenge. The amount of olive oil used ina challenge was only a drop. The olive oil was purchased from a local grocery store or possibly from the commissary at Fort Detrick and was not ordered through USAMRIID's purchasing system. [_____——C~CNC#dsSttat'ed that he always used glass bottles of olive oil and could not recall ever using a plastic bottle. indicated that the bottles of olive oil were small. ne Do e of olive oil was kept on the cold side of Building in room and another bottle stored on the hot side in the glassware cabinet in room Extra bottles of olive oil were not maintained by the erobiology group in Building[ | It has been stated that when olive oil was used in the aerosol challenges, it was added without prior mixing with any other solution. A 1 ml plastic pipette was used to remove olive oil directly from the bottle. One drop of olive oil was added to the material in the AGI. [.___———sidJ noted that one (1) bottle of olive oil lasted for years before being emptied; however, bottles occasionally disappeared. Antifoam was taken from the laboratory by other personnel much more often than was the olive oil. ICE: Ice was used frequently in the process of handling the material used in the aerosol challenges. Samples were typicall maintained in ice baths prior to_use. Ice machines in room and in the hallway between rooms andL__] in Building were the sources of the ice used. Commercially purchase never used. If the machines in Building[___|were not functioning, the technicians used one of the machines in Building ice was ooo oF op mEN Sd uy QO b6 b7c