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FD-302a (Rev. 10-6-95) b6 279A-WF-222936-USAMRIID b7C Continuation of FD-302 of ,On 04/07/2004,Page _ said that the same AgIs have been used for at least the past ten (1o) years. Recently the AgIs have been modified to use a screw top instead of a push on top. The material that was collected in the AgI would be. maintained for several days or longer following a challenge.. Small amounts of the antifoam were aliquoted into tubes.. In previous years, larger tubes were used which caused excess waste.. noted that Anthrax spores are very resilient is unaware of any antifoam stock ever reported missing. said that would assume that knew the process of using antifoam as part of the aerosol challenges. in the past olive oil was used for aerosol challenges. The olive oil was used for challenges involving toxin. proteins. Olive oil has not been used during challenges for ten (1o). years. Thas not seen a bottle of olive oil at USAMRIID since the early 1990's. stated that there is no difference in the post challenge. assays if olive oil or antifoam are used during the aerosol challenge.