◆ SpookStack

To: Laboratory From:. Washington Field Re: 279A-WF-222936, 11/01/2005 On 08/02/1997, completed the fifteenth b6 fermentation production run, which resulted in 20oml of 3.4x1010 b7C spores/ml (approximately 6.8xlo12 total spores). During dilutions both before and after heat shock procedures no Bacillus globigii contamination was noted.. On 08/04/1997, lcompleted the sixteenth. fermentation production run, which resulted in 18oml of 3.lx1010 spores/ml (approximately 5.6x1012 total spores). During dilutions before heat shock procedures three (3) colonies of Bacillus globigii contamination were noted on one (1) dilution plate. On 09/09/1997, completed the seventeenth fermentation production run, which resulted in 17oml of 4.6x1010 spores/ml (approximately 7.8x1o12 total spores). During dilutions before heat shock one (1) colony of Bacillus globigii contamination was noted on one (1) dilution plate at two (2) separate dilutions, and after heat shock procedures one (1) colony of Bacillus globigii contamination was noted on one (1) dilution plate. On 09/15/1997, completed the eighteenth fermentation production run, which resulted in 18oml of 2.7xl010 spores/ml (approximately 4.9x1ol2 total spores). During dilutions after heat shock procedures three (3) colonies of Bacillus globigii contamination were noted on one (1) dilution plate. On 09/23/1997, completed the nineteenth. quantity and an unknown concentration. No Bacillus globigii contamination was noted during the concentration assay. The disposition of this fermentation run is unknown, however, 70ml of irradiated spores, concentration 5.Oxlo' spores/ml, dated 09/23/1997 were recovered in a consensual search of the Lothar Dugway, Utah on 06/30/2004. Summary of Bacillus globigii Contamination. Bacillus globigii contamination was noted in five. productions runs dated 06/28/1997, 07/21/1997, 08/04/1997, 09/09/1997, and 09/15/1997. All five (5)of these runs were sent to Ivins at UsAMRIID of which three (3) were added to a stockpile compiled by Ivins call RMR 1029. Additionally, four (4) productions runs, (04/18/1997, 06/23/1997, 07/01/1997, 07/29/1997) were aborted and autoclaved due to Bacillus globigii. contamination. There is no explanation in notes as to a 0505a.ec 4