◆ SpookStack

1. To: Laboratory From: Washington Field Re: 279A-WF-222936, 11/01/2005 for Anthrax Spore Preparation in Bench Top Fermentors.". b6 b7C On 03/25/1997, received four (4) 1ml. polypropylene tubes containing Bacillus anthracis Ames for use in fermentor production runs. On 04/07/1997 began the first. spore production run of the 19 total runs that would be initiated for shipment to Ivins/usAMRIID between 04/23/1997 and 09/03/1997. Summary of Fermentation Production Runs On 04/12/1997, completed the first fermentation production run, which resulted in 6.3xlo total spores or 70ml of 9.0xiolo spores/ml. During dilutions both before and after noted. On 04/14/1997, completed the second fermentation production run, which resulted in 9.5xloi total spores or 150ml of 6.3xlol0 spores/ml. During dilutions both before and after heat shock procedures no Bacillus globigii contamination was noted. On 04/18/1997, aborted the fermentation run that began on 04/15/1997 due to Bacillus globigii contamination. According to notebook this batch was autoclaved on 04/18/1997. On 04/22/1997, completed the fourth fermentation production run, which resulted in 7.0xloi total spores or 280ml of 2.5xlo10 spores/ml. During dilutions both before and after heat shock procedures no Bacillus globigii contamination was noted. On 04/25/1997, completed the fifth fermentation of 3.Oxlolo spores/ml. During dilutions both before and after heat shock procedures no Bacillus globigii contamination was noted. On 05/19/1997, completed the sixth fermentation production run, which resulted in 4.8xioi2 total spores or 120ml of 4.Oxloi0 spores/ml. During dilutions both before and after heat shock procedures no Bacillus globigii contamination was noted. On 05/22/1997, completed the seventh. fermentation production run, which resulted in 5.3xiol total spores or 155ml of 3.4xlolo spores/ml. During dilutions both 80505a.ec 2