◆ SpookStack

To: Laboratory From: Washington Field Re: 279A-WF-222936, 11/01/2005 on 08/02/1997, [____]completed the fifteenth be fermentation production run, which resulted in 200ml of 3.4x10*° _ spores/ml (approximately 6.8x10 total spores). During dilutions both before and after heat shock procedures no Bacillus globigii contamination was noted. On 08/04/1997, [______]Jcompleted the sixteenth fermentation production run, which resulted in 180ml of 3.1x10*° spores/ml (approximately 5.6x10* total spores). During dilutions before heat shock procedures three (3) colonies of Bacillus globigii contamination were noted on one (1) dilution plate. On 09/09/1997,[ ____—|completed the seventeenth fermentation production run, which resulted in 170ml of 4.6x10*° spores/ml (approximately 7.8x10" total spores). During dilutions before heat shock one (1) colony of Bacillus globigii contamination was noted on one (1) dilution plate at two (2) separate dilutions, and after heat shock procedures one (1) colony of Bacillus globigii contamination was noted on one (1) dilution plate. On 09/15/1997, [_______Jcompleted the eighteenth fermentation production run, which resulted in 180ml of 2.7x10?° spores/ml (approximately 4.9x10¥ total spores). During dilutions after heat shock procedures three (3) colonies of Bacillus globigii contamination were noted on one (1) dilution plate. On 09/23/1997,[ _| completed the nineteenth fermentation production run, which resulted in an unknown quantity and an unknown concentration. No Bacillus globigii contamination was noted during the concentration assay. The disposition of this fermentation run is unknown, however, 70ml1 of irradiated spores, concentration 5.0x10’ spores/ml, dated 09/23/1997 were recovered in a consensual search of the Lothar Salomon Life Sciences Test Facility at the Dugway Proving Ground, Dugway, Utah on 06/30/2004. Summary of Bacillus qglobigii Contamination Bacillus globigii contamination was noted in five productions runs dated 06/28/1997, 07/21/1997, 08/04/1997, 09/09/1997, and 09/15/1997. All five (5)o£ these runs were sent to Ivins at USAMRIID of which three (3) were added to a stockpile compiled by Ivins call RMR 1029. Additionally, four (4) productions runs, (04/18/1997, 06/23/1997, 07/01/1997, 07/29/1997) were aborted and autoclaved due_to Bacillus globigii contamination. There is no explanation in[ si notes as toa 0505a.ec 4 LE