◆ SpookStack

—, To: Laboratory From: Washington Field Re: 279A-WF-222936, 11/01/2005 to produce spores for Ivins under the project entitled "Procedure for Anthrax Spore Preparation in Bench Top Fermentors." On 03/25/1997, [____] received four (4) iml polypropylene tubes containing Bacillus anthracis Ames for use in fermentor production runs. On 04/07/1997 began the first spore production run of the 19 total runs that would be initiated for shipment to Ivins/USAMRIID between 04/23/1997 and 09/03/1997. Summary of Fermentation Production Runs on 04/12/1997,[ ___completed the first fermentation production run, which resulted in 6.3x10*" total spores or 70ml of 9.0x10*° spores/ml. During dilutions both before and after heat shock procedures no Bacillus globigii contamination was noted. On 04/14/1997,[____]completed the second fermentation production run, which resulted in 9.5x10** total spores or 150ml of 6.3x107° spores/ml. During dilutions both before and after heat shock procedures no Bacillus globigii contamination was noted. On 04/18/1997,[_______Jaborted the fermentation run that began on 04/15/1997 due to Bacillus globigii contamination. According to[__—sijnotebook this batch was autoclaved on 04/18/1997. On 04/22/1997,[______] completed the fourth fermentation production run, which resulted in 7.0x10” total spores or 280ml of 2.5x107° spores/ml. During dilutions both before and after heat shock procedures no Bacillus globigii contamination was noted. On 04/25/1997,[___] completed the fifth fermentation production run, which resulted in 7.5x10” total spores or 250ml of 3.0x10?° spores/ml. During dilutions both before and after heat shock procedures no Bacillus globigii contamination was noted. On 05/19/1997,[___]completed the sixth fermentation production run, which resulted in 4.8x10¥ total spores or 120ml of 4.0x107° spores/ml. During dilutions both before and after heat shock procedures no Bacillus globigii contamination was noted. On 05/22/1997,[_______]completed the seventh fermentation production run, which resulted in 5.3x10™ total spores or 155ml of 3.4x10?° spores/ml. During dilutions both [__P0s0sa.ec 2 oe ~~ OF Od