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‘ To: Washington u® From: Washington Field Re: 279A-WF-222936-USAMRIID, 1/11/2006 4/12/2001 Exposure 01-040, née 0-03 ech: Bic b7E Ba Ames Stock RMR 1029 Room[ sd Hood 1 & 8 27 Rabbits exposed Aerosol logs show that a total of 55 animals were exposed during this part of study BOO-03. Protocol BO01-07 Title: Evaluation of Antibiotic Treatments against Bacterial Biological Warfare Agents (anthrax, plague, glanders) in Mice. Objectives: Susceptibilities to current, and many new or experimental antibiotics, have been established in vitro for B. anthracis and B. mailei in our laboratory and this screening continues (attached manuscripts). Antibiotic MICs for Y. pestis are currently being determined. The true test of the effectiveness of any antibiotic is the ability to contribute to a successful treatment in an infection model. The working hypothesis is that if B. anthracis, B. mallei or Y. pestis were used ina biowarfare/terrorist situation these microorganisms would most likely be resistant to the current antibiotics that are designated for treatment. The objective of this protocol is to identify additional antibiotics that could be used as alternate treatments should resistance to current treatments occur. Agents: Ames strain of B. anthracis Registry No. 2244 6/26/2001 Exposure 01-065, Protocol BO1-07 PI: Tech: Ba Ames Registry No. 2244 Room[L___} Hood 1, 60 Mice exposed 1 x 107, 1x 105, 1 x 108, 1 x 107, 1 x 10%, and 1 x 10° Note: Antifoam was utilized as part of this . aerosol challenge. There is_no record in bé Ivins' logs to indicate that| hws bie given any of RMR 1029 prior to 10/4/2001. 7/9/2001 Part 4 of multi-part aerosol study (BO00-03) 50 ml used Note: It is assumed that this 50 ml was used for the 7/10/2001, 7/11/2001, and 7/12/2001 aerosol challenges. 19