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Amerithrax — Part 21
Page 20
20 / 29
:
FD-302a (Rev. 10-6-95)
279A-WF-222936-USAMRIID, 279A-BA-C101392
Continuation ofFD-302 of
,On 05/08/2003
,Page
b6
b7C
biological samples internally to personnel at usamRiid.
Idid not
grow
]used in the studies, rather
interfaced with a
person growing the virus in
group at
USAMRIID.
received samples
from
who
assisted
significantly with sample handling..
also
for use in the project.
In addition to supplying
samples for the project
provided training to
Iaboratory protocols in a
received initial
training from
of USAMRIID
spent no more than 40 hours in the
spent a
little more time than
Initially, a commercial mass spectrometer was used to.
obtain the biological signatures of myriad agents. An organism in
liquid form was deposited on a slide.' Once dry, the slide was
measurements were made in
laboratory space.
Extension of the project, to include
required the construction.
of
a
Once
was
taken to UsAMRIID for testing.
laboratory
was used to conduct the aerosgl work.
group worked with
laboratory
personnel,
mainly
Agents
were
]worked mainly with Bacillus anthracis. BRuce IvINS
provided
with a tube of liquid B.a. Ames spores at a.
b6
concentration of ~1012 cfu/mL.
ldid not know the specifics of
b7C
how the spores were grown, but recalled that the material was
b2
gradient purified using renograph. No additives were used to
b7F
enhance the properties of the spores. The Ames spore preparation
was stored in the
]walk-in cooler in the containment.
facility of Building
The tape samples containing deposited.
agents were stored in a Tupperware container in the same location.
did not
recall the maintenance of a log or inventory for the
walk-in cooler.
was aware of the location and types of.
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