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FD-302a (Rev. 10-6-95) 279A-WEF-222936 The Dugway samples were maintained b only used the Dugway material for This study was prior to the The samples were stored in E believes some of the Dugway Ames was agar grown. CL JDugway material was kept in the original orange screw-ca containers and was put in the refrigerator in lab when was finished with it. endor£— tubes in the The sheep's blood agarL________Jused in study was made forL___]by the Tissue Culture group at USAMRIID. did not wear gloves when preparing the plates and setting up the lab for the work. The broth is only opened in the hood long enough to withdraw some with a pipette. The initial streaking of the agar plate is very brief, and the bacteria is not exposed for long. Some people believe the entire batch of spore preparation is heat shocked at the same time, but[___————_sd IVINS only heat shock the portion needed when they are ready to do each challenge. IVINS would usually go into the lab early and heat shock the amount needed for the aerosol challenges. also heat shocked to et_ rid of vegetative cells and possibly to stimulate the_bacteria. diluted down to get the target 100 microliters from[___] stock solution_and put it in a first dilution tube. That tube would be the one heat shocked. The material was freshly heat-shocked each time. used that makes a nice density gradient (one could use sucrose). IVINS had been using the whern[ __—_—i| came to USAMRIID, and[__] continued to usé is willing to take the agents on a tour of the USAMRIID laboratories where [__]|worked if necessary. provided a copy of the published article which is maintained in the 1A section of the file.