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. FD-302a (Rev. 10-6-95) @ 279AR-WE-222936 bé b6 bIC bIF The finished product is used in intra-muscular (IM) challenges. For aerosol studies, the product is further processed with a density gradient to get the_vegetative cells and other be matter out. None of the material for yvaerosol bic challenges was agar-grown. does not think e material at was grown on agar. has not grown Ames spotres on agar. The Ames sporulates very well. If left in an incubat¢r for 24 hours, the Ames will have some spores. stored the Ames spores in 50 ml conical tubes in, b6 the of suite[__] The Ames in these tubes was for the IM bIC challenges and was not highly purified. The ultra-pure Ames for the aerosol challenges was stored in one-liter poly flasks and was kept separate from_the IM challenge material. All of the material was stored in the[__koom, but may also have some of the Ames in the unlocked refrigerator in Room Each tube was labeled with the name of the strain, Che date of harvest, and the concentration. Two flasks would produce enough’ material for the guinea pig work. estimated that the concentration.of spores was something like 2 x 10° or 10° per ml. When the colony is initially re-suspended_in 5 ml, traces of the sheep's blood agar are not visible. did not document each individual tube of Ames spores prepared, and it would have been possible for someone to take some of the spore prep without | Jnoticing. [—___]eninks[_Jxesearch group is the only group b6 at USAMRIID to grow Bacillus anthracis on agar. bIC ~1T OY ro