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FD-302a (Rev. 10-6-95) 279A-WEF-222936-USAMRIID Continuation of FD-302 of Bruce Edward Ivins ,On 05/07/04 , Page 2 The Ames strain IVINS has was originally obtained from IVINS does not know what happened to the second slant and infected tissue sample from the original shipment, and he never saw them. To IVINS knowledge, no one at USAMRIID has found those two parts of the original shipment of Ames to USAMRIID. ssa[__] advised IVINS that she is in possession of his laboratory notebooks and had questions about some of the notations. IVINS provided the following: "L-broth" is not Leighton-Doi broth. It is a media composed of yeast extract, beef extract, and sodium chloride, which produces little spore formation with Ames. When Leighton-Doi broth was used, it would have been noted as "L-D broth" of Leighton-Doi. IVINS recently told another FBI agent that they make the final resuspension of spores in water for injection (WFI), which is endotoxin-free bottled water, but he was wrong. After his conversation with the agent, he asked and learned that they now do the final resuspension in USAMRIID house-distilled water which has gone through a deionizing column. They also use this water to make Leighton-Doi broth for growing Ba. IVINS has never had to add antifoam or any other chemical to his production method for producing Ames spores. The shaking of the shaker flasks in the broth production is not enough to create a need for antifoam or any similar product. He is not aware of any protocol used at USAMRIID which calls for the use of antifoam with Ames. The protocol for the aerosol challenges does not call for antifoam. IVINS showed sAs[ sian the difference between Ames grown on agar and Ames grown in broth. They use broth-grown material for all of the challenges because the Ames preparations made that way are so clean in comparison to agar- grown material. IVINS does not recall ever sending Ames to BIOPORT. In 2001, he sent Ames spores to BMI. IVINS fo went to a conference on -exposure prophylaxis problems. At this conference, Pd (phone tie) of BMI talked about Ames which belonged to PERRY MIKESELL. MIKESELL could have taken Ames to BMI, but it should have been documented. IVINS has no knowledge of MIKESELL discreetly taking Ames _fr USAMRIID to BMI. If MIKESELL did so, he should not have. [| may have more information about MIKESELL's Ames.