Amerithrax — Part 29

123 pages · May 08, 2026 · Document date: Nov 19, 2001 · Broad topic: Terrorism · Topic: Amerithrax · 115 pages OCR'd

‹

FD-302a (Rev. 10-6-95) cre) OQ ro 279A-WF-222936 Continuation of FD-302 of Bruce Edwards Ivins on 2/29/02 “Page 2 inventory of all cultures maintained by the Bacteriology Division at USAMRIID. , Ivins obtained the Ames strain of B. anthracis from [| sometime between 1981 and 1983. Ivins obtained this strain to use as a challenge strain. Ivins did not regularly begin using Ames in his research until[———~—C_] Showed that the Ames strain was very "hot" in 1996. Prior to that time, Vollum 1B was the standard strain that was used in Ivins research. Full genetic characterization has not been done on all strains used in the Bacteriology Division, however, Ivins does know the properties of most of the strains, including the biochemical characteristics as well as virulence factors. Regarding the origin of the Ames strain, Ivins recalls obtaining Ames for the first time in late 1980, possibly late December or in early 1981. Ivins has reviewed notebooks to find the first reference to the Ames strain. The earliest reference that Ivins found to Ames was in 12/81. Ivins found nothing in the notebooks prior to that date regarding Ames. Ivins provided a hand drawn diagram detailing the genesis of the Ames strain at USAMRIID. Ivins showed that he obtained B. anthracis from in 1985 which Ivins in turn shared with From USAMRIID Ivins also sent out samples of this strain to Dugway Proving Grounds, the Defense Research Establishment Suffield (DRES), the University of New ico, and Battelle. Ivins_is aware that provided strain, referred to as who in turn supplied it to Porton Down in Great Britain, and tol Louisiana State University who in turn supplied the strain to the University of Northern Arizona. The method of propagation of the Ames strain of B. anthracis differed between and Bruce Ivins. Ivins used single colony dilutions to propagate the Ames culture. used mass swipes of culture to propagate his collection. As a result, mutations became evident in collection of the strain that were not present in Ivins' strain collection. Ivins has documentation and records to back up all shipments of the Ames strain which he has sent out. Ivins will provide isolates of the B. anthracis he has in his collection if needed by the FBI. Ivins noted that spore samples are usually maintained in a one percent phenol at USAMRIID

›

FD-302a (Rev. 10-6-95)

cre)

279A-WF-222936

Continuation of FD-302 of Bruce Edwards Ivins on 2/29/02 “Page 2

inventory of all cultures maintained by the Bacteriology Division
at USAMRIID.

, Ivins obtained the Ames strain of B. anthracis from
[| sometime between 1981 and 1983. Ivins obtained this strain
to use as a challenge strain. Ivins did not regularly begin using
Ames in his research until[———~—C_] Showed that the Ames
strain was very "hot" in 1996. Prior to that time, Vollum 1B was
the standard strain that was used in Ivins research. Full genetic
characterization has not been done on all strains used in the
Bacteriology Division, however, Ivins does know the properties of
most of the strains, including the biochemical characteristics as
well as virulence factors.

Regarding the origin of the Ames strain, Ivins recalls
obtaining Ames for the first time in late 1980, possibly
late December or in early 1981. Ivins has reviewed
notebooks to find the first reference to the Ames strain. The
earliest reference that Ivins found to Ames was in 12/81. Ivins
found nothing in the notebooks prior to that date regarding Ames.
Ivins provided a hand drawn diagram detailing the genesis of the
Ames strain at USAMRIID. Ivins showed that he obtained B.
anthracis from in 1985 which Ivins in turn shared with
From USAMRIID Ivins also sent out samples of this
strain to Dugway Proving Grounds, the Defense Research

Establishment Suffield (DRES), the University of New
ico, and Battelle. Ivins_is aware that provided
strain, referred to as who in turn

supplied it to Porton Down in Great Britain, and tol
Louisiana State University who in turn supplied the strain
to the University of Northern Arizona.

The method of propagation of the Ames strain of B.
anthracis differed between and Bruce Ivins. Ivins
used single colony dilutions to propagate the Ames culture.

used mass swipes of culture to propagate his collection.
As a result, mutations became evident in collection of
the strain that were not present in Ivins' strain collection.

Ivins has documentation and records to back up all shipments of the
Ames strain which he has sent out.

Ivins will provide isolates of the B. anthracis he has in
his collection if needed by the FBI. Ivins noted that spore
samples are usually maintained in a one percent phenol at USAMRIID