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coordination of common suite duties (such as cleaning)[ was the supervisor for many years, but-net recently. and was recently renamed B3 supervisor by the Chief of Bacteriology Division. b6 bic Q. Was the intensity of contamination described by_vhen she did the surveillance cultures inside the lab after the incident on April 8 heavier than you would find when surveillance cultures used to be done? A. NeThe total intensity of the contamination was greater and also of special concern, since spores were | found on the inside passbox latches as well as on shoes and articles of clothing in the "hot" changerooms. We When conducting surveillance in the past, we would sometimes find a few hot spots of contamination and clean them up, then re-check to make sure they were OK. Q. Did you report your concerns about cleaning to anyone? A. I have believe that L mentioned it of fn the past. I've also informally talked with other investigators in the Bacteriology Division about cleaning and cleanliness issues. Usually, In the past, if people people have had specific concerns about suite cleaning or cleanliness, they -raised the issues in a general way-of cleanliness-in-generaland-(as well as the need for improvement) at Division meetings, but no specific individuals were are mentioned at those meetings. Q. Have there been any other concerns about cleanliness? A. A few letters came into the Bacteriology Division for analysis during Operation Noble Eagle. The DSD was concemed that at least seme one of the samples they had sent to be tested were-being was contaminated in room Q. Who coordinateds the sample processing in room__| cS a also have-worked in there. Normally it is a. landers research laborato Q. What live agents comes in and out of the B-3 area? A. For anthrax aerosol challenges spray, we used-te send spores out through the passbox_in a hard plastic transport container to be taken to Bldg 1412. Aerosol challenges are done there, dose is confirmed, and data are crunched in 1425. Anthrax AGI samples does not come back as they formerly did several years ago, since the AGI cultures are now done in 1412 by_______ and me, Plague AGI may come back. Q. How are spores prepared? . Cultures are grown up in shaker flasks. They a are often centrifuged in 500 ml tubes bottles in a large (GS-32?GSA-er-G-3) rotor. The Rrotor is too big to take to the hood, so the tubes bottles are usually removed from the rotor in the centrifuge. Q. Are there any secondary containers in that rotor, like sealed cups? A. No. Sometimes the tube a bottle breaks and you can hear a slurping sound when the bottletubes are-is removed, Q. Does the centrifuge have a floor hood or other containment system? A. No. There used to be a system to evacuate the air in the centrifuge through a filter in case there was a break, but that hasn’t been used in years. Q. That wouldn’t help if there was a broken tube, would it? A.No. Q. Has the ventilation system worked OK as far as you know? Are there magnehelic gauges at the clean room entry? A. I don’t think the suite has ever gone positive. I have never noticed the magnehelic readings in the entry. The gauge between the office area and the hallway are barely negative. Q. What are the procedures to follow if you have a potential exposure to B. anthracis? pO