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To: Washington Field From: Washington Field Re: 279A-WF-222936-USAMRIID, 05/24/2005 remaining 25 colonies derived from IVINS' cultures of April 15- 16, 2002. Of the 27 colonies, 20 were typed as the Ames strain, four were typed as the Vollum 1B strain and two were typed as the b2 Sterne strain. No product was obtained from the analysis of one b7F of the 27 colonies. The 27 colonies represented B.a. contamination in only three locations. Of the 15 colonies obtained from room (IVINS' office), 10 were Ames strain, two were Sterne strain, two were Vollum 1B, and one yielded no product during PCR analysis. Of the six colonies derived from the cold-side of the B3 pass-box, all six typed as Ames. Of the six tested from the men's B3 change room, four were B.a. Ames, and two were B.a. Vollum 1B.% [WRITER'S COMMENTS - Following is a summary of the environmental survey results, incorporating IVINS' data with that obtained in the follow-up surveys. Between April 15-25, 2002, approximately 1197 samples were collected from over 100 locations. Of the 1197 samples, 16 cultures tested positive for the presence of virulent b6 B.a. Two of the B.a. cultures derived from the USAMRTTD-wide bic survey, while the remaining 14 derived from IVINS' surveys on pe April 15-16, 2002. IVINS plated a total of 51 samples, 27% of TE which yielded virulent B.a. colonies. Only two of the 1141 samples, or 0.18%, derived from the surveys conducted in response to IVINS' independent swabbing, yielded virulent B.a. colonies. Of the over 100 sites surveyed in Building 1425, positive results for the presence of virulent B.a. were obtained in only three locations: the_men's change room a the cold-side B3 pass- box, and room Notably, these were e three sites originally surveyed by tws Pos surveys, only the office shared by IVINS, | ee ee tested 1 positive for the presence of virulent B.a.j ° Comparison between IVINS' survey and the USAMRIID-wide survey When asked why he was able to find virulent B.a. when the other swabbing efforts did not, IVINS explained that he utilized a more aggressive and extensive method for collecting samples. According to IVINS, he sampled a larger area, especially hard to reach and dusty areas, and used more force. D6 He also explained that prior to the J ing survey —_ by CHPPM, took be samples outside the laboratory and subsequently cleaned the DIE area thoroughly with each.°? IVINS stated that he believed there were additional areas that were contaminated; however, everyone had cleaned their areas as a preventative measure. IVINS stated that the random sampling conducted by USAMRIID safety staff consisted of about 6-10 swabbings in high traffic areas, such as the phone, desk, and computer. He felt the technique was inadequate. IVINS asserted that he took : — Cc omen ~1 OS