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‘. To: Washington Field From: Washington Field Re: 279A-WF-222936-USAMRIID, 05/24/2005 areas, support areas and pass-boxes outside all laboratories. Samples were acquired from the Building 1425 break room and 22 and On April 19, 2002, a total of 838 samples were collected from 86 sites within the building. A total of 107 non-hemolytic, bacillus-like (NHB) colonies were obtained from the 838 samples. On April 20-21, 2002, personnel from the Center for Health Promotion and Preventative Medicine (CHPPM) of Aberdeen Proving Ground, Maryland, conducted additional sampling. CHPPM Survey Set #1 included the laundry center at the Jeanne Bussard Center and the DIS equipment. A total of 58 samples were collected during CHPPM Survey #1 on April 20, 2002. Five NHB colonies were obtained from five sites. On April 21, 2002, CHPPM Survey Set #2 included sampling_in the following areas: Bl, the B2/B3 hallway, the_pass-box tol] the men's clean change room , and zoon[ A total of 113 samples were collected from nine sites within e building during CHPPM Survey #2. Of the 113 samples, nine NHB colonies were obtained. On April 23-25, 2002, supplemental surveys of the Budget office, room and room were conducted. Of the 65 total samples collected, two sites yielded a total of two NHB colonies.” e Results of USAMRIID-wide microbiological survey Of the 1074 samples collected during the environmental surveys on April 19-25, 2002, a total of 123 NHB colonies were isolated from 102 sites. The 123 NHB colonies were identified as Bact #1 through Bact #123.°° The NHB colonies were subjected to determination of sensitivity to gamma phage and to PCR analysis for the presence of genes for capsule and protective antigen (PA). O£ the 123 total NHB colonies, only two colonies were confirmed positive for the presence of both B.a. capsule and PA genes. Notably, the two positives were obtained in locations where positive results were previously reported following IVINS' independent survey. The two colonies, identified as Bact #115 and #116, were confirmed positive for virulent B.a. and were later genotyped as Ames. Bact #115 was obtained during CHPPM y Set #2 and was described as "C-99, clean-side change room, locker top." Bact #116 was obtai during CHPPM Survey Set #2 and was described as "C-78, rm. bookcase, rt, inside door." On May 8, 2002, the genotypes of 27 B.a. colonies derived from the surveys were reported. Two of the colonies resulted from the USAMRIID-wide microbiological survey, while the : a 1 OF