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oe @ Té: Washington Field From: Washington Field Re: 279A-WF-222936-USAMRIID, 07/14/2004 anthracis (Ba) Sterne, a protocol for the challenge of anthrax polysaccharides, anthrax polysaccharide protein assays, and other similar information. Entries were made by[ and an unknown individual. On page 1, dated October 1, 1980, an unnamed growth media recipe was provided for isolation and purification of olysaccharides from Bacillus sp., requirin On_page 10, written January 19, 1981, an experimental protocol by was listed. This protocol was entitled "Challenge of 'anthrax polysaccharide - Sterne strain! vaccinated guinea pigs with virulent B. anthracis, Vollum 1B." The objective of this protocol was to determine whether vaccination of guinea pigs with polysaccharide derived from the attenuated B. anthracis, Sterne strain would protect the animals against challenge with the virulent Vollum 1B strain of B. anthracis. No results were immediately provided. An April 27, 1981 entry on page 15 provided a recipe for "G" medium in which Sterne could be sporulated: A recipe for "R" medium was listed on page 17 and dated April 28, 1981. Page 27, written by an unknown individual but initialed _and dated by[__] revealed that on July_1i5, ee up and ran an ELISA test with chel strain of anthrax spores. On page 32, spore coat protein studies were conducted using the following medium: Another unknown individual's handwriting was displayed on pages 42-44, and involved developing an ELISA test for the PA oO bic ro ~1 OY ol bé bIC