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+ ws ) FD-302a (Rev. 10-6-95) 279A-WF-222936-USAMRIID bé Continuation of FD-302 of [ , On 03 / 28 / 2003 , Page 3 b7c [ ___]zeceiveal 1 stock of B.a. Ames from BRUCE IVINS. IVINS went back to the original B.a. Ames slant and generated a new stock in order to keep the pathogen as close to the host as possible. IVINS provided his Ames strain to[___]in the following manner: He retrieved his stock from the freezer, took out gave the plate to In addition to the B.a. Ames This material was generated from IVINS' stock To maintain uniformity of Lineage among the laborator everyone already Investigators in the Bacteriology Ames_stock. was not sure of the origin strains [~~] does not store much in the way of 'true' Ames stocks or spore preparations. It would be fairly obvious if vials were missing. However, someone could take a loop out without notice. With the small volumes of Ames that stores, if an appreciable volume was gone, [___]would notice. stores numerous stocks of Ames variants. These materials are visually distinct from wild type Ames. 'Normal' Ames appears pale gray, with medusa-like colonies, when plated and is not hemolytic. With aging, the culture becomes grainy and blebs appear. Ames mutants appear yellow in color and display hemolytic activity. If a person did not know better, he might mistake the mutant material as not anthrax. their own Ames stocks. Division used IVINS! of the Ames used by various B.a. Ames. has never had a reason to of B.a. Ames spores. enerate a large amount