◆ SpookStack

FD-302a (Rev. 10-6-95) 279A-WF-222936-USAMRIID Continuation of FD-302 of a CY 17/2003 .Page 4 Each cryovial of primary stock in the archive contained approximately 0.5 mL of material. A secondary stock of a given B.a. strain was generated from the corresponding primary stock; however, most of the vials in the archive were never used in this manner. To generate a secondary stock of a particular strain, a loop of approximately 10 microliters (vL) was pulled from cimary stock and cultured on either agar or broth media. never worked with the original Ames slants and was not aware of how the Ames original slants were received by USAMRIID. never saw any of the original Ames materials and was not sure 1f the primary stocks in the archive were derived directly from the original slant. 1 no knowledge of the B.a. Ames stocks generated by BRUCH IVINS and believed that IVINS was gone by the time that arrived at USAMRIID and thought that he gave his B.a. Ames stocks tof described in detail the procedures for the shipping of pathogenic samples. The guidelines differed for the transport of virulent and avirulent organisms. For requests of virulent materials, such as B.a. Ames, regulations were followed, as defined by the Select Agents Program of the CENTERS FOR DESEASE CONTROL AND PREVENTION (CDC). To receive Select Agents, the/requester had to be registered with the CDC. Prior to shipment of €he pathogenic materials, transfer paperwork, or EA1O1 documents, were processed through the appropriate channels.