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*BD-302 (Rev. 10-6-95) FEDERAL BUREAU OF INVESTIGATION ALL INFORMATION CONTATNED HEREIN Is UNCLASSIFIED DATE 12-15-2008 BY 60324 UC BAU/DK/TH Date of transcription 06/12/ 2003 bé On June 6, 2003, date of birth b7c at MEDICAL RESEAR STITUTE OF INFECTIOUS DISEASES (USAMRIID), PORTER STREET, FORT DETRICK, MARYLAND (MD), 21702, telephone by Special Agents (SAs)[7—— ______JAND After being advised of the identities of the interviewing Agents and the purpose of the interview,[__] provided the following information: | Jat USAMRIID, Was quéried regarding microorganism decontamination h : ; technologies and /ftheir effects on subsequent analyses. telephone telephone participated in the discussion. Sodium hypochlorite, a primary component of household bleach, denatures proteins via oxidation. Destruction of bacterial spores and degradation of DNA result from contact with ~5-103 household bleach. DNA has been detected using polymerase chain reaction (PCR) techniques pursuant to treatment of bacterial spores with bleach. Hydrogen peroxide is a strong oxidizer, known to cleave peptide bonds. DNA AWAY, a caustic oxidant produced commercially, also destroys proteins and DNA. The effectiveness of decontamination with bleach, DNA AWAY and similar commercial products may be reduced if additional organic molecules and proteins are present along with the nficroorganisms. These additional molecules may bind up the decontaminating agent, thus hindering interactions with the target microorganisms. Protein denaturation is the primary mechanism of inactivation by formaldehyde gas. Specifically, formaldehyde interacts with amine groups and affects carbamide linkages. Positive hits with PCR have been obtained after decontamination; however, a detectable PCR signal could require ridding th b7E Investigation on 06/06/2003 at Fort Detrick, Maryland ce) NOY, Fk? 279R-WF-222936-USAMRIID 279A-BA-C101392 Date dictited SA by This document contains neither recommendations nor conclusions of the FBI. It is the property of the FBI and is loaned to your agency; it and its contents are not to be distributed outside your agency.