◆ SpookStack

Federal Bureau of Investigation Re: Interview on 31 March, 2005 1) Emails in the September, 2001 through October 2001 timeframe. Those emails should be in the computer information that Special Agen got from my office computer. There are many emails in that timeframe in the "OLD FOLDERS AND FILES" folder. 2) Request for the first subpoena (early 2002) asking for our stocks. | gave the subpoena to 3) The first materials sent to the Repository. | gave to Special Agent____lthe information. I'm sorry that | didn't clarity that "Dugway Ames Spores - 1997" is also known as (is the same as) RMR 1029. The material was also known as "GLP Ames spores," even though they actually weren't technically GLP quality. As | related to you yesterday, | honestly don't remember who actually streaked the slants Caen myself), whether the slants were directly inoculated from the stocks or from sheep blood agar plates. If the latter, ] don't know specifically how many colonies were used to inoculate each slant. | regret thatthel___| spore stocks were overlooked in the first materials provided to the repository. 4) I-1 strain. This was provided if sd We found it in the refrigerator in my lab in B313, and it was put into the FBI lock box in the B3 coldroom. 5) | talked over the information on the calculations on the concentration of RMR 1029 spores with We performed more plate counts on the material yesterday afternoon, and we read the plates this morning. He collected the data. | also gave to him data and information related to past determinations on the concentration of the RMR 1029 spores. He also took possession of the remaining amount (about 2.5 mi) of the RMR 1029 spores. 6) Where the flasks of RMR 1029 were kept. Since we had a lab (room 115) in Building 1412 at the time, and since the spores were intended for aerosols, it's possible that at least one of the flasks was kept in the lab refrigerator in 115 or in the 1st floor coldroom (much less likely) for a certain amount of time. We were eventually - | think it was probably before 2001 - “moved out" of the area by Aerobiology, and at that point may have brought RMR 1029 material back to 1425. | honestly don't remember, but it would make sense. 7) Time period that we helped BioPort with their vaccine problems. The time period for helping BioPort was April, 2000 into the fall of 2001. 8) Lyophilized spores. | related to you yesterday that frozen spores, once thawed, look very poor. They clump and have lost considerable refractility. When materials such as proteins, viruses or vegetative bacterial cells is lyophilized, a "cryoprotectant" such as glycerol, albumin, sucrose, DMSO, etc. is used to keep the material from being damaged during freezing and thawing. | don't know if your scientists have looked for the presence of a cryoprotectant in the evidence, but that might be a reasonable idea. - Bruce Ivins