◆ SpookStack

A NFORMATION CONTATHED eS} i N IS UNCLASSIFIED | DATE la-ll-2008 BY 60324 uc bauw/re/ Analysis of Sample Date analyzed - 23 October, 2001 Date of Report - 24 October, 2001 Sample___SPS02.88.01 | received the sample (in a microcentrifuge tube in a ziplock bag) from on the afternoon of 23 October, 2001. The tube was weighed, and it was estimated to contain about 0.01 g of material. In B-3, 0.99 ml of sterile water for injection was added to the material. After thorough mixing, the material was transferred to a second tube. The original tube was disinfected with bleach, dried and weighed. The net weight of the granular material was determined to be 0.0145 grams. Ten-fold dilutions were plated out onto TSA, then incubated overnight. Plate counts were made, and it was determined that the original tube contained 1.93 X 10° CFU per ml. Since there was 0.0145 grams of material, this calculates to be 1.33 X 10‘! CFU per gram of powder material. Visual inspection of the suspension of material under phase contrast microscopy found few (<5%) visible vegetative cells, a small amount (<10%) visible debris, and very few small clumps. Most of the material appeared to be individual refractile spores. Interpretations and conclusions: If this is a preparation of bacterial spores, itis a relatively pure preparation.. This preparation did not appear as pure as the material (SPS02.57.03) previously examined on October 17, 2001. The SPS02.57.03 preparation contained spores at a concentration of 2.1 X 10"? per gram. The SPS02.57.03 spores were thus approximately 15.8 times "hotter" or more concenirated per gram than the SPS02.88.01 spores examined here. Bruce E. Ivins, Ph.D. . USAMRIID Bacteriology Division ace O font we