◆ SpookStack

bo SA 70 t FD-302 (Rev. 10-6-95) 5bA -1 - FEDERAL BUREAU OF INVESTIGATION ALL INFORMATION CONTAINED HEREIN IS UNCLASSIFIED DATE 12-15-2008 BY 60324 UC BAW/DK/TH Date of transcription .06/12/2003 b6 On June 6, 2003, date of birth b7C Social Security Account Number was nterviewed at place of employment, the UNITED STATES ARMY MEDICAL RESEARCH INSTITUTE OF INFECTIOUS DISEASES (USAMRIID) PORTER STREET, FORT DETRICK, MARYLAND (MD), 21702, telephOne by Special Agents (SAs) AND After being advised of the identities of the .. interviewing Agents and the purpose of the interview, provided the following information: b6 b7C at USAMRIID, was queried regarding microorganism decontamination. technologies and /their effects on subsequent characterization. analyses. telephone and telephone also participated in the discussion. Sodium hypochlorite, a primary component of household bleach, denatures proteins via oxidation. Destruction of bacterial spores and degradation of DNA result from contact with ~5-10%. household bleach. DNA has been detected using polymerase chain reaction (PcR) techniques pursuant to treatment of bacterial spores with bleach. Hydrogen peroxide is a strong oxidizer, known to. cleave peptide bonds. DNA Away, a caustic oxidant produced commercially, also. destroys proteins and DNA. The effectiveness of decontamination with bleach, DNA AwAy and similar commercial products may be reduced if additional organic molecules and proteins are present along with the microorganisms. These additional molecules may bind up the decontaminating agent, thus hindering interactions with the target microorganisms. Protein denaturation is the primary mechanism of. inactivation by formaldehyde gas. Specificaliy, formaldehyde. interacts with amine groups and affects carbamide linkages.. Positive hits with pcR have been obtained after decontamination; however, a detectable pcR signal could require ridding the-sample b2 302 b7E Y N Investigation on 06/06/2003 at Fort Detrick, Maryland 8181 Fiie# 279A-WF-222936-USAMRIID. 279A-BA-C101392' Date dictated SA by SA This document contains neither recommendations nor conclusions of the FBI. It is the property of the FBI and is Ioaned to your agency; it and its contents are not to be distributed outside your agency.