◆ SpookStack

FD-302a (Rev. 10-6-95) 279A-WF-222936-USAMRIID, 279A-BA-C101392 b6 b7C Continuation of FD-302 of ,On_04/29/2003 ,Page 2 b6 obtained wet B.a. Ames spores for the. experiments from. b7C IVINS. Panbtt tog uoaybgnt ut saaods dy? Math Aaoaenoqet .SnIaI b2 media, using a shaker and a three day incubation period in the b7F dark. b6 b7C b7F At the conclusion of the experiments,. autoclaved the B.a. Ames spores. obtained from. IVINS. The spores for the project were obtained from Ivins and were grown in LB broth at 37'c in a small flask. Antibiotics were used to select clones with the GFp plasmid. The Ames stock obtained from Ivins was autoclaved. made spores using non-virulent B.a. strains. The. production method depended on the strain being used. SBA plates were, used most often; however, B.a. Sterne was grown using