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mem A NFORMATION CONTAINED M IS UNCLASSIFIED FD-302 (Rev. 10-6-95) DATE 12-09-2008 BY 60924 C Bal/sRS/VCF -1- FEDERAL BUREAU OF INVESTIGATION Date of transcription 01/04/2006 ROS SSSR account was interviewed on D er 19, pee 1425 stare Route 142 ‘Si sectal “agents (SNS) and Also present/during the interview was Assistant General Counsel, Battelle. After Si re) he identities of the interviewing Agents and the purpose /of the interview, was presented with an AMERITHRAX; Major Case-184 Non-disclosure Agreement (NDA). After reviewing the document, signed the NDA. At this point,[__] provided the following information: Lois currently a has worke attelle fora a oe Bacillus anthracis (Ba) Ames strain sample received from Dr. Line, spre of USAMRIID, — some of the fermeht6r rung “performed at Battelle. During these fermentor runs, it became evident that the cultures were at Battel contaminated. suspected that the cultures were contaminated due to the fact that had extra difficulty getting the culture to | ores. The contamination of the cultures was confirmed when exami mounts made from the cultures under a Microscope. believes that examined the wet mo twenty-four (24) and forty-eight hour time points. isolated and did some characterization of the contaminant by first growing the culture in tryptic soy broth (TSB) and then streaking it onto sheeps blood agar (SBA) or brain heart infusion agar (BHI). believes that the contaminant was mucoid and may have hemolytic on SBA. Biochemical tests (API strip) performed_b indicated that the contaminant was Bacillus pumilus. developed a standard operating procedure (SOP) for enrichment_of the contaminant and agreed to provide this SOP to the FBI. [sd believes that any purified isolates of the contaminant that may have been generated have probably been destroyed. Investigation on 12/19/2005 at Columbus, Ohio File # 2°79A-WF-222936-BATTELLE — Date dictated N/A This document contains neither recommendations nor conclusions of the FBI. It is the property of the FBI and is loaned to your agency; it and its contents are not to be distributed outside your agency. does not communicate wi RIID directly, but does not | loz. | |