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FD-302a (Rev. 10-6-95) 279A-WE-222936, 279A-BA-C101392 be Aerosol Challenges using B.a. Ames spores were conducted against test animals, including guinea pigs and rhesus macaques. The effectiveness of USAMRIID'S trial vaccines was compared to that of the Anthrax Vaccine Adsorbed (AVA), the licensed human anthrax vaccine. The development of an anthrax vaccine necessitated tion among numerous USAMRIID scientists, includin a did not w the virulent B.a. Ames spores used in the studies, nor did participate in the aerosolization of the spores. The B.a. Ames spores were generated by IVINS, | Jan nown to interviewing Agents as media in shake flasks. A special centrifuge with a vacuum was u roduction process a minimal media supplemented with bicarbonate, was developed by IVINS [recalled using blood agar on occasion and involvement in a study which employed Bacillus subtilis. never produced B.a. Ames spores. [ did not receive _or send Select Agents during [ tenure at USAMRIDD.[—] IVINS,[ | shipped