◆ SpookStack

To: Washington ®.. From: Washington i Re: 279A-WF-222936, 05/21/2004 EC. After performing some calculations, Ivins estimated that, to achieve the spore concentration found in the anthrax letter mailings, approximately 300 milliliters of the Dugway RMR 1029 material would have been required. This amount of missing material would have been noticed. In a December 12, 2003 interview, Ivins indicated that he had approximately 100 milliliters left of this sample; however, it is unclear whether Ivins is basing this statement on his records or on the actual sample. Given the subtraction error (see page 4) made by Ivins. on February 22, 2000, it is possible that Ivins may actually have 200 milliliters left of the Dugway RMR 1029 sample. Ivins did not notice this subtraction error until it was pointed out to him during an FBI interview. The Ames produced under this agreement was further processed at USAMRIID by Ivins as described ina separate EC. It was then used in live form by Ivins and other USAMRIID researchers in anthrax vaccine challenges. Also contained within Notebook standard operatin rocedure (SOP), entitled This documen ined the cultures and the processing methods to be used for preparation of Ba spores in less than 10-liter quantities. Such preparations _ were typically utilized as challenge material in testing bio- b6 defense material. The preparations could also be employed for ”’ other applications. For example, the spores could serve as antigens for the production of antiserum or could be used as a coating antigen in antibody capture enzyme-linked immunosorbent assays (ELISAs). This draft SOP_is presumably, but not conclusively, the method Dugway_used to for usanerzD; [—"___Jeent an e-mail on February , 1998 requesting that a letter stating that the draft SOP was the final procedure used to make the USAMRIID spore preparations. However, no such letter from was found in the notebook. One of the steps in the above SOP under "Materials and Methods" required the addition of DIE