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@ ° @ \ * FD-302a (Rev. 10-6-95) 279A-WEF-222936-302 Continuation of FD-302 of ee ,On 09/23/2004 _ ,Page 2 [___| The construction of aerosolization capabilities are underway at For a iven biological agent, material was typi produced noted that the last batch of laboratory. As an example, generated in| laboratory lasted Typically, approximately milliliters (mL) of material, with a concentration of 106-107 organisms, were generated. The batch of bacteria was aliquoted and used in all subsequent studies. According tol using material from the same production run decreased the number of variables to consider in a group of experiments. In preparation for an animal challenge, an aliquot of material was diluted. estimated that 5,000 to 10,000 organisms were enough to expose hundreds of animals. Once a vial of material was accessed for dilution, any residual material in the original tube was discarded after the experiment. did not prefer the agar plate growth method, as "a lot of extra junk" was present in the spore preparation when the bacteria was grown on agar plates. Agar plates were used in laboratory only for titering purposes. received the Ames strain of Bacillus anthracis (B.a.) from BRUCE IVINS of the United States Army Medical Research Institute of Infectious Diseases put in contact with IVINS through noted that well at that time. was of USAMRIID knew each other did not recall the exact date of the shipment; however estimated that the material was received some time around speculated that IVINS sent ~1 mL of 108 Ames spores in one screw-top tube.[__ | believed that the spores were frozen. During the interview, [__]located the EA101 documenting the transfer of B.a. Ames from IVINS. The EA101 indicated that one tube, containing 500 microliters L) of spores at 3 x 10°/mL, was shipped to[_____ Jon [| notified the Centers for Disease Control and Prevention (CDC) regarding receipt of the material, as was not confident that USAMRIID personnel would file the appropriate paperwork. roup did not access the material sent by IVINS until The original material was aliquoted into multiple vials. Hach vial was labeled as "0S1," denoting that ~1 OV omen Q