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@ . » S ., FD-302a'(Rev. 10-6-95) 27 9A-WF-222936-USAMRIID Continuation of FD-302 of Bruce E. Ivins ,0on09/08/2004 page 33 likely to clump, and aerosolize better. [| (phonetic) b7c told IVINS that he had difficulty aerosolizing spores grown on agar and that broth grown spores aerosolize better. IVINS indicated that there would be additional information about the "1989" sample in his notebooks. He stated that the "1989" sample was not currently in use at USAMRIID. IVINS created at third seed stock in "2002" by plating a spore from the "1981" original slant onto blood agar and | ) Tt is this "2002" seed stock that is a in use. IVINS noted that he is making nearly 1 trillion spores a week for USAMRIID, the National Institute of Allergies and Infectious Diseases (NIAID), the Centers for Dis rol (CDC), etc. As an example, IVINS explained that currently conducts aerosol challenges using spores at the followin ersons were making spores at USAMRIID: CY IVINS did not know if these persons inoculated plates using a sweep technique or using a single spore technique. Prior to knowledge of the two plasmids, USAMRIID was mostly interested in Ba toxin production. IVINS noticed that newer cultures (those created more recently) produced more toxin that older cultures. He did not recall if anyone was investigating Ba spore virulence as affected by multiple passages of that organism at that time. IVINS did notice that Ba samples which were grown using the single spore technique produced more toxins than those grown using the sweep technique. IVINS described RMR 1030 as a “bunch of spores" grown by IvINS[L_—sidin 1995 and 1996. Nearly.all of RMR 1030 has been used. The only remaining RMR 1030 has been provided to the FBI. IVINS reviewed notebook # 3655, page 72, dated 3/8/96. This notebook described the creation of RMR 1030 and noted that Standard Operating Procedure (SOP) "UIB-BI-3" was used to grow RMR 1030. This material was used for aerosol challenges of rabbits and possibly NHPs. IVINS was working with IVINS then printed a copy t 989" sample was the seed stock for any material made under SOP UIB-BI-3, to include RMR 1030. UIB-BI-3 was initially a challenge protocol for which he