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United States Patent: 6,316,006 @ @ Page 1 of 10 USPTO PATENT FULL-TEXT AND IMAGE DATABASE ALL INFOREATION CONTAINED Images HEREIN IS UNCLASSIFIED smages DATE 12-15-2008 BY 603924 UC BAW/DE/TH (28 of 145) United States Patent 6,316,006 Worsham, et al. November 13, 2001 Asporogenic B anthracis expression system Abstract This invention relates to a bacterial expression system for production of protective antigen (PA) against bacillus anthracis. Recombinant asporogenic B. anthracits that are derived from .DELTA.Sterne-1 (pPA102) and show inability to bind the dye when grown on Congo Red Agar can be screened and asporogenic strains isolated using methods of the invention. organisms of the invention lacking spore- forming function may be killed by heat shock at temperatures as low as 60,degree. C. for 60 minutes. Hence, contamination of the environment with viable spore-forming organisms is easily avoided and decontamination is easily accomplished. Inventors: Worsham; Patricia (Jefferson, MD); Friedlander; Arthur M. (Gaithersburg, MD); Ivins; Bruce (Frederick, MD) Assignee: The United States of America as represented by the Secretary of the Army (Washington, DC) Appl. No.: 346238 Filed: November 23, 1994 Current U.S. Class: 424/246.1; 435/252.3; 435/252.31; 435/485 Intern'] Class: C12N 015/00 Field of Search: 424/246.1 435/172.1,172.3,252.3,252.31,200.1 References Cited [Referenced By] Other References Ivins et al. Abstr. Gen. Meet. Am. Soc. Microbiol. 94 meet. 150, May 1994.* Ivins et al. Infect. Immun. Feb. 1990. 58(2): 303-308.* Ivins et al. Infect. Immun. Nov. 1986. 54(2): 537-542.* Ivins et al. Infect. Immun. May 1986. 52(2): 454-457. http://patft.uspto.gov/netacgi/nph-Parser?Sectl=PTO2&Sect2=HITOFF &u=/netahtml/sear... 6/28/2005