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Tod: Washington Field From: Washington Field Re: 279A-WF-222936-USAMRIID, 07/14/2004 would take pictures of the results. On October 22, 1997, Ivins wrote another e-mail to all the above individuals except In this e-mail; he discussed dispensing the spores be into various aliquots, and revealed that the concentration was biG ee ~ Another notebook entry was entitled "Scope of Work - Bacillus anthracis Ames Spores". A letter was sent from[___] ne RES MRSS SBOESE and detailed the terns of EHS agreement involving USAMRIID shipping 4 vials of Ba spores to P Dugway. Dugway would then grow a total of approximately 10 batches of spores, which would be sent as individual shipments to USAMRIID. The USAMRIID points of contact were designated as Bruce Ivins (technical) and administrative), while the Dugway points of contact were (administrative) and. _———édw (technical). A standar operating procedure (SOP) containing the details of how the spores were produced, equipment used, materials, and quality control procedures used was to be provided within one month after Dugway's last shipment to USAMRIID. In-addition, batch records for each spore preparation produced would contain the following quality control results: the percentage of spores and vegetative cells, a test for the viability of spores after heat-shocking of a test sample, electron microscopy photographs of spore preparations, photographs of colony morphology, photographs of spore stains, and phage sensitivity. In addition, 10 colonies from each batch would be tested on halo agar. The supplier, lot number of media components used, media sterility test results, and the results of purity testing of the spore preparations would also be provided. Purity testing was to be conducted prior to heat shocking. Each batch, including all photographs and results, was to include a batch number at the time of the shipment. The batch records were to be sent within two weeks of each shipment. Also contained within the notebook was a Dugway procedure for anthrax spore preparation in bench top fermentors. This standard operating procedure (SOP) draft outlined the culture and processing methods used for preparation of Ba spores in less than 10 liter quantities. Such preparations were for use as challenge material in testing bio-defense material. The preparations could also be used for other applications such as antigen for the production of antiserum or for use as coating antigen in antibody capture enzyme-linked immunosorbent assays (ELISAs) . On February 12, 1998, e-mailea[__|to request that Dugway send a letter stating that the draft SOP