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Amerithrax — Part 13
Page 108
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yaa _ LL Fogel NFORMATION ATHED
5 ha emantaomte ero sonn omemen ser em BarE TI b)-2008 BY 60324 UC paises jut
SPORE PREPARATION FORM
STUDY #: )D99-02
DATE: 15 OCT 99 SPORE PREPARER: Ivins
SPORES USED: B. anthracis Ames, RMR 1029, in 1% phenol
APPROXIMATE CONCENTRATION OF SPORE STOCK SOLUTION: 2.5 X 10"/ml
DESIRED CONCENTRATION OF SPORES FOR AEROSOL| _|
DILUTION FACTOR: I ml of spores pe] dof aerosol suspension
NUMBER OF ANIMALS TO BE CHALLENGED _|
AMOUNT (ML) OF SPORE SUSPENSION NEEDED FOR asrosoL{ |
AMOUNT OF SPORES ADDED TO BOTTLE: _}oicrotites
AMOUNT OF WATER ADDED TO BOTTLE: [fu
SPORES DELIVERED TO BE AEROSOLIZED AT: 0730
PLATE COUNTS (FROM| —_ DILUTION):
PLATE 1 PLATE 2 PLATE 3 PLATE 4 PLATE 5
ZO 2! SG 2¢ 3.2
AVERAGE= 257,
CONCENTRATION OF BACTERIA IN SUSPENSION FOR AEROSOL:
OTHER REMARKS:
SIGNATURE OR INITIALS: bLA DATE: lo 13/99
DATA VERIFIED BY: | | DATE: Av Cu ¥ f
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