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| © . = | INFORMATION CONTAINED HE IS UNCLASSIFIED DATE le-10-2000 BY 60924 00 BAW sSRS/LSC SPORE PREPARATION FORM STUDY #: B00-03 (part 4; 1 dose efficacy with 1, 5, 25 and 100 pg PA per dose) DATE: 10 July 01 SPORE PREPARER: Ivins SPORES USED: B. anthracis Ames (RMR 1029) be APPROXIMATE CONCENTRATION OF SPORE STOCK SOLUTION: 3.9 X 10°/ml DESIRED CONCENTRATION OF SPORES FOR AEROSOL, | DILUTION FACTOR: 1 ml of spores pe, __ pf aerosol suspension NUMBER OF ANIMALS TO BE CHALLENGED: |__| AMOUNT (ML) OF SPORE SUSPENSION NEEDED FOR AEROSOL: AMOUNT OF SPORES ADDED TO BOTTLE: __| AMOUNT OF WATER ADDED TO BOTTLE, | SPORES DELIVERED TO BE AEROSOLIZED AT: morning of 10 July 01 PLATE COUNTS (FROM, —_ PILUTION): PLATE1 PLATE 2 PLATE 3. PLATE4 PLATES Hl) 23 4 2% 28 AVERAGE= 39, 3 | ACTERIA IN SUSPENSION FOR AEROSOL: OTHER REMARKS: SIGNATURE OR INITIALS: pate: // J, /@] DATA VERIFIED BY: DATE: /7 7 hy Os on