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FD-302a (Kev. 10-6-95) @ : 2 279A-WF-222936-USAMRIID bo b7C Continuation of FD-302 of oe Co 8/2008 »Page 2 At usamerrD, [| __|was familiar with a5 1, 20 1, anda 100 1 fermentors. The 100 ermentor (actual 80 (1) working volu intained in the laboratory suites in Building DIC 1425. indicated that it was never used other than on b2 occasion water _was_run through it just to ensure that it was still bIE operational.[__] explained that when using the 20 1 fermentor, it took one day for the fermentor to complete the growth cycle for Delta Stérn Ba. [ __Jaavises that the 5 1 fermentor, when operational, did not producé a whole lot of noisef[__lopined that the easiest way to have produce the volume of Ba used in the anthrax-laced letter mailings would have been to use shaker flasks as opposed to using fermentors.[ _]expounded that using the shaker flask method to grow Ba would have been much less conspi use shaker flasks are always in use at USAMRIID anyway advised that 8 one liter flasks could be run at the same time in a shaker incubator. believed that there is one shaker incubator, known in IVINS laboratory and a second shaker incubator, known as "Big Bertha," in the[__] suites. When using the fermentors| __| recalled that it was Standard operating procedures when growing Ba to use anti-foam in bé order to reduce the buildup of b foam that would occur b7c during the fermentation process. explained that anti- foam would be automaticall umped into the fermentor to reduce the amount of punbles.[ [recalled that the old brand name for the anti-foaming agent was Called Mazu and believed that Mazu may have been sold out or taken over by a newer company possibly called Sigma. does not recall IVINS having any discussions concerning either fraternities or sororities. recalled that IVINS o es around the office. IVINS IVINS was explained that an easy target due Tlot Sx Py picked o on IVINS pecause IVINS was suc