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the 2001 timeframes — This water was pyrogen-fres and contained To: Washington Field From: Washington nicl Re: 279A-WF-222936-USAMRIID, 11/16/2004 system. The Cell Culture Group collects the penthouse water in large carboys (on an as needed basis), and utilizes the water for solid and liquid media preparations. [ CCC_| stated that water purchased from Baxter Internationa b6 ; ncorporated was used for * aerosol challenges and post-challenge plate dilutions during BIC no endotoxins. The aerosol challenge area gets deionized water from Building 1412's distribution system, with Millipore filtration’ units attached to several faucets for further purification. According = the Cell Culture Group provided the sterilize sed in the aerosol challenges. Bruce Ivins would sometimes provide the —-——g terre watery; which s’ purchased ‘from-a~commercial~source ™ “ """" - ; and stored in Suit in Building 1425. Purchased water was used, specifically, in the all glass impingers (AGT) in aerosol challenges. f provided mek on = core water for USAMRIID. 8 ort Detrick operates its own water treatment plant which purifies raw water from the Monocacy bé River. The treatment plant which utilizes sand filtration, b7C has not changed its method of treatment except for the discontinuation of adding fluoride starting in approximately 1996.[__——saiso provided annual reports of regulated contaminant monitoring results from 1996 to 2003. team stated that Building 1412 and 1425 have independent water purification systems. Building 1412 utilizes steam to distill water, and Building 1425 utilizes . reverse osmosis (RO). Neither building has had eas to © the systems since its inception. (Since th interview, upgrades to the purification systems have been implemented in both Building 1412 and 1425.) Each laboratory has its own separate filtration units to provide an extra layer of purification. Many of these units are Millipore, Aqua Solutions, or Biocel brands. There are different models of each brand, but they all function simj by running the water through several layers of liters. [| provides a list of all the water ations units currently in the USAMRIID laboratories, and provided the locations of each one. °